Maximum likelihood trees inferred from the ddRAD data matrices based on (a.) de novo assembly and (b.) reference assembly method against Operophtera brumata genome (GCA_001266575). Bootstrap support values are indicated above the branches and only the values > 50% are shown. The barplot shows the assignments of individuals into two genetic clusters, the green clusters referring to Perizoma incultaria, the orange clusters to P. barrassoi. Each bar represents one individual and colors represent the proportion of the individuals that belong to each of the genetic cluster. Samples infected with Wolbachia are marked with an asterisk.