Research Article |
Corresponding author: Zdenko Tokár ( zdeno.tokar@gmail.com ) Academic editor: Jadranka Rota
© 2017 Zdenko Tokár, Aleš Laštůvka, Erik van Nieukerken.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Tokár Z, Laštůvka A, van Nieukerken EJ (2017) A new pygmy leafmining moth, Stigmella tatrica sp. n., from the alpine zone of the Tatra Mountains (Lepidoptera, Nepticulidae). Nota Lepidopterologica 40(1): 131-140. https://doi.org/10.3897/nl.40.12674
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Stigmella tatrica sp. n. is described from moths taken in the alpine zone of the Tatra Mountains in Slovakia. The new species is similar to several other montane species of Stigmella Schrank, 1802 in the S. aurella (Fabricius, 1775) group in external characters and male genitalia; its closest relative is S. dryadella (O. Hofmann, 1868). It is indistinguishable from S. tormentillella (Herrich-Schäffer, 1860) by the colour and pattern elements of the forewing; however, it differs in the male genitalia in the shape and number of cornuti, in the female genitalia by long apophyses with protruding ovipositor, and by COI barcodes. Immature stages are unknown, but Dryas octopetala L. (Rosaceae) is the possible hostplant. The long ovipositor suggests an unusual, possibly hidden place for oviposition. The male and female adults and genitalia of both sexes are figured and photographs of the habitat are provided.
The pygmy leafmining moths or Nepticulidae have around 300 species in Europe, the great majority of which are leafminers, feeding on woody plants and they are abundant in forested areas (van
The high altitude Stigmella species of the Tatra Mountains, especially above the tree line, have been poorly studied and all records reside in a handful of publications. In the Polish part of the alpine zone Stigmella species were first observed by
In late July 2005, while inventorying the Lepidoptera fauna of the Western Tatra Mountains, the first author with his friend found four males of an unknown Stigmella species in alpine grasslands at altitudes between 1800 and 1900 m. Detailed study of two specimens by the second author was inconclusive, because of the relatively high similarities of their external appearance and male genitalia to closely related Stigmella species in the S. aurella group.
Later on, additional males were found in the eastern part of the Tatras, Belianske Tatra Mts at altitudes of ca. 2000 m. These specimens were sent to the third author for DNA analysis. In 2016 the first author’s last visit to the locality brought success in the form of a large series of specimens, including two females. Individuals of the new species were observed flying around the low cover of high-altitude plant communities with Dryas octopetala L. An examination of the female genitalia of this tiny moth, as well as the analysis of the DNA barcodes, showed that it represents a hitherto undescribed Stigmella species.
All specimens were taken as adults during daylight, either using a net or caught directly from the leaves of Dryas octopetala into small glass vials. The genitalia were dissected in the usual way for small Lepidoptera, the preparations being stored in glycerol in small plastic vials or embedded in Euparal on glass. The drawings of the male and female were made by the second author using water colours. Drawings of the genitalia were made by the first author using Indian ink on transparent sheets. Photographs of the type locality were taken using digital cameras Canon PowerShot G11 and Nikon Coolpix P600.
For methodology of DNA barcodes we refer to papers of the third author and his colleagues (van
The nomenclature of species follows the “Revised classification and catalogue of global Nepticulidae and Opostegidae” (van
AL Aleš Laštůvka
BOLD Barcode of Life Database
Gp Genitalia preparation
ZT Zdenko Tokár
Holotype: ♂, pinned, with genitalia in glycerol in a small plastic vial. Original labels: “Slovakia, Belianske Tatry, Zadné Jatky, 1950–2010 m, 49°14.18’N; 20°13.50’E, 30.vii.2016, Zdenko Tokár leg.”, “HOLOTYPE Stigmella tatrica Tokár, Laštůvka & van Nieukerken” (red label), coll. Z. Tokár (to be deposited in the Central Slovakia Museum Banská Bystrica).
Paratypes: 14♂, 2♀, same locality and data as holotype, Gp. ZT ♂ 12873–5, 12878, 12918, ♀ 12876, ZT leg., coll. ZT & AL; 1♂, František Kuraj leg. & coll.; same locality as holotype, 2.viii.2014, 2♂, Gp. ZT 12266–7, ZT leg., coll.
Adult, male (Fig.
Female (Fig.
Male genitalia (Figs
Female genitalia (Fig.
Stigmella tatrica is somewhat similar to several other montane species in the Stigmella aurella group in forewing colour and pattern and in male genitalia. Externally it is indistinguishable from S. tormentillella (Herrich-Schäffer, 1860). Carpathian S. dryadella differs from the new species by more glossy forewings, a wider violet area before fascia and paler head and collar. In the male genitalia the new species is most similar to S. geimontani, S. stelviana and S. aeneofasciella, but it differs significantly from these externally. Unlike S. tatrica, S. tormentillella and S. dryadella have only one large cornutus in their vesica. In the female genitalia S. tatrica differs considerably from other species in the group by the long and strong apophyses, forming a protruding ovipositor.
The early stages of the new species are unknown. The adults were observed flying over low cover of high-altitude plant communities with the presence of Dryas octopetala and resting or quickly moving on leaves of that plant. Adults were collected during daylight hours between 29th July and 4th August (light collecting was not attempted). This likely represents a single annual generation.
(Figs
The Belianske Tatra Mountains and the Red Mountains are both karst areas, built of limestone and dolomite with a dominance of subalpine or alpine grasslands with many different plants growing there, many of which are endemic, rare or endangered species. The vegetation at the type locality could belong to the Caricion firmae Gams association, with the following higher plant species commonly present: Carex firma Host (Cyperaceae), Dryas octopetala L. (Rosaceae), Androsace lactea L., Primula auricula L., Soldanella carpatica Vierh. (Primulaceae), Arenaria tenella Kit., Dianthus nitidus Waldst. & Kit., Silene acaulis (L.) Jacq. (Caryophyllaceae), Bartsia alpina L., Pedicularis oederi Vahl. ex Hornem., P. verticillata L. (Orobanchaceae), Bellidiastrum michelii Cass., Crepis jacquinii Tausch (Asteraceae), Bistorta vivipara (L.) Gray (Polygonaceae), Campanula cochleariifolia Lam. (Campanulaceae), Chamorchis alpina (L.) Rich. (Orchidaceae), Festuca versicolor Tausch (Poaceae), Galium anisophyllon Vill. (Rubiaceae), Pinguicula alpina L. (Lentibulariaceae), Ranunculus alpestris L. (Ranunculaceae), Saxifraga aizoides L., S. caesia L., S. paniculata Mill. (Saxifragaceae), Selaginella selaginoides (L.) P. Beauv. ex Schrank et Mart. (Selaginellaceae); the following mosses: Ctenidium molluscum (Hedw.) (Schimp.) (Hylocomiaceae), Ditrichum flexicaule (Schwaegr.) Hampe (Ditrichaceae), Tortella tortuosa (Hedw.) Limpr. (Pottiaceae); and the following lichen: Cetraria islandica (L.) Ach. (Parmeliaceae) (
The specific name tatrica, an adjective, is derived from the Tatra Mts, where the new species was discovered.
(Fig.
Maximum likelihood tree of European species of the Stigmella aurella group, showing the position of Stigmella tatrica in red. Small figures represent bootstrap values, after 100 replicates. Bootstrap values below 50 are not given. A specimen of Stigmella tityrella is used as outgroup. Nomenclature follows van
The discovery of Stigmella tatrica shows that the montane fauna of Central European Nepticulidae is still insufficiently studied and can provide surprises. We expect that S. tatrica can also be found in the Polish part of the Tatra and other parts of the Carpathians, e.g. in Romania; currently it seems to be an endemic species for the Carpathians. Although the partial mitochondrial gene COI as used for DNA barcodes is usually not sufficient for a robust phylogenetic analysis, both analytical methods invariably group S. tatrica with S. dryadella, and this together with the fact that S. tatrica has usually been collected on or near Dryas octopetala, suggest that the two species are sister taxa whose ancestor shifted to Dryas. The protruding ovipositor suggests that S. tatrica has an unusual, possibly hidden oviposition site, and potentially rather different leafmines compared to S. dryadella, or even a different feeding mode.
Our thanks are due to Ignác Richter (Malá Čausa, Slovakia) and František Kuraj (Krompachy, Slovakia) for providing the specimens and accompanying the first author during his research trips in the Western and Belianske Tatra Mts, the latter also for taking the photographs of the habitat. Tomasz Jaworski (Raszyn, Poland) provided necessary literature. Zdeněk Laštůvka (Brno, Czech Republic) and Camiel Doorenweerd (Leiden, the Netherlands) are acknowledged for their advice on the manuscript. The latter and Frank Stokvis (Leiden, the Netherlands) performed the DNA analyses. Marko Mutanen (Oulu, Finland), Peter Huemer (Innsbruck, Austria), and Andreas Segerer (München, Germany) kindly gave permission to use DNA barcodes from their BOLD projects. We thank David Agassiz (Weston-super-Mare, UK) for linguistic editing.