Research Article |
Corresponding author: Geir Søli ( geir.soli@nhm.uio.no ) Academic editor: Bernard Landry
© 2018 Geir Søli, Leif Aarvik, Trude Magnussen.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Søli G, Aarvik L, Magnussen T (2018) Plutella polaris Zeller, 1880 (Lepidoptera, Plutellidae) rediscovered at Svalbard, Norway, with comments on its taxonomic position. Nota Lepidopterologica 41(1): 119-127. https://doi.org/10.3897/nl.41.22423
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After 142 years, Plutella polaris Zeller, 1880 was rediscovered at Svalbard, Norway, in 2015. The locality and its vegetation are described. The specimen is the first female ever recorded, and its genitalia are illustrated. The taxonomic position of P. polaris relative to other Plutella Schrank, 1802 species in the Northern hemisphere is discussed.
In 1873, seven specimens of an unknown microlepidopteran species were collected by the entomologist Reverend A.E. Eaton at Wide Bay, Svalbard, Norway, between 21st July and 24th July. The specimens were subsequently handed over to Henry Tibbats Stainton, who at the time was Britain’s leading authority on Microlepidoptera. Stainton sent two of the males to his German colleague Philipp Christoph Zeller for his opinion. In a letter to Stainton dated May 29th 1874, Zeller described the species and named it Plutella polaris.
Since the species was first discovered in 1873, no further records had been reported until 9 July 2015, when a female was collected by Geir Søli at Ringhorndalen, Wijdefjorden, Svalbard. The Ringhorndalen specimen (Fig.
In the present paper, we provide supplements to the description of the species and its habitat at Svalbard, along with an updated account of its taxonomic status.
Ringhorndalen is part of the Indre Wijdefjorden National Park and one of several valleys on the eastern side of the Wijdefjorden. Wijdefjorden is the longest fjord on Svalbard, being 108 km long and cutting deep into Spitsbergen from the north (Fig.
As Wijdefjorden is not easily accessible during summer months, its flora has just recently been more thoroughly investigated (e.g.
The single specimen of P. polaris was found on a south facing slope in the inner part of the valley (79.3358°N 16.1289°E) (Fig.
The Lepidoptera fauna of Svalbard is very poor, with three resident species only: P. polaris, Apamea exulis (Lefèbvre, 1836) and Pyla fusca (Haworth, 1811) (
Presently 26 species of Plutella Schrank, 1802 are recognised worldwide, of which a few must still be regarded as dubious (see
Species | Distribution |
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Plutella armoraciae Busck, 1912 | Nearctic: North-western North America |
Plutella geniatella Zeller, 1839 | Palaearctic: Central Europa |
Plutella haasi Staudinger, 1883 | Palaearctic: Norway, Sweden, Scotland |
Plutella huemerella (Baraniak, 2007) | Palaearctic: France: Alpes-Maritimes |
Plutella hyperboreella Strand, 1902 | Holarctic: Norway, Sweden, Finland, Canada, Alaska |
Plutella kyrkella (Baraniak, 2007) | Palaearctic: Russia: SW Altai |
Plutella mariae Rebel, 1923 | Palaearctic: Novaya Zemlya |
Plutella notabilis Busck, 1904 | Nearctic: North-western North America. |
Plutella polaris Zeller, 1880 | Palaearctic: Norway and Russia (Altai) |
Plutella porrectella (Linnaeus, 1758) | Holarctic: North America and Eurasia |
Plutella xylostella (Linnaeus, 1758) | Cosmopolitan |
Of the seven syntypes originally designated for Plutella polaris, only two males are still extant in the Natural History Museum, London. What has happened to the remaining five syntypes is not known. In addition to our rediscovery of P. polaris in Wijdefjorden, we were recently informed of another specimen identified as P. polaris. This specimen, a single male, was found during an expedition by Austrian and Russian lepidopterists to the Republic of Altai in Russia in 2016 at a considerably lower latitude of 49.5°N, 88.08°E (
Compared to other species of Plutella that occur in northern Europe, the forewing pattern of P. polaris (Fig.
As the female of P. polaris is found for the first time, a short description and illustration of the female genitalia (Fig.
In order to confirm the species identity of P. polaris, and derive more information about its taxonomic position relative to other Plutella species in the Northern hemisphere, we performed an analysis based on DNA barcoding. One leg was sampled from the Wijdefjorden specimen and sent for DNA extraction, amplification and sequencing at the Canadian Centre for DNA Barcoding in Guelph. The DNA barcoding fragment of the mitochondrial cytochrome c oxidase subunit 1 (CO1) was sequenced, using standard primers and bi-directional Sanger sequencing. The DNA sequence and original trace-files of the P. polaris specimen from Svalbard are publicly available in the dataset ‘Plutella polaris Svalbard, Norway’ (DS-PPSN) (doi: dx.doi.org/10.5883/DS-PPSN) in the Barcode of Life Data System (BOLD) (
In addition to the CO1 sequence obtained from P. polaris, 41 CO1 sequences from nine other species of Plutella were obtained from BOLD (
The final dataset consisted of 52 nucleotide sequences, with 552 positions (Table
As can be seen from the Maximum likelihood tree (Fig.
List of specimens included in the DNA barcode analysis together comprising 52 nucleotide sequences. Abbreviations used for depositories:
Species | BOLD Process ID | Country | Depository |
Plutella hyperboreella | LEFIA800-10 | Finland |
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Plutella hyperboreella | LEFIA801-10 | Finland |
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Plutella hyperboreella | LEFIC108-10 | Finland |
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Plutella hyperboreella | MNAG269-08 | Canada |
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Plutella hyperboreella | MNAG268-08 | Canada |
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Plutella haasi | LEFID118-10 | Russia, N Ural |
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Plutella haasi | LON5958-17 | Norway |
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Plutella notabilis | MNAJ558-09 | Canada |
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Plutella huemerella | PHLAB606-10 | Italy |
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Plutella geniatella | PHLAA575-09 | Italy |
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Plutella geniatella | PHLAA094-09 | Italy |
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Plutella geniatella | PHLAB255-10 | Italy |
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Plutella geniatella | PHLAB256-10 | Italy |
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Plutella geniatella | PHLAI835-13 | Italy |
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Plutella porrectella | LEFIJ911-10 | Finland |
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Plutella porrectella | RRGCO094-15 | Canada |
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Plutella porrectella | LEFIJ910-10 | Finland |
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Plutella porrectella | LEFIK544-10 | Finland |
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Plutella porrectella | LON5956-17 | Norway |
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Plutella polaris | LEALT906-16 | Russia, Altai |
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Plutella polaris | LON2736-16 | Norway, Svalbard |
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Plutella armoraciae | NGNAQ667-14 | Canada |
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Plutella armoraciae | NGAAF282-14 | Canada |
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Plutella armoraciae | SMTPI9177-14 | Canada |
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Plutella armoraciae | MNAJ560-09 | Canada |
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Plutella armoraciae | MNAK560-10 | Canada |
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Plutella xylostella | LEFIB587-10 | Finland |
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Plutella xylostella | LEFIB955-10 | Finland |
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Plutella xylostella | LEFIJ1709-13 | Finland |
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Plutella xylostella | LON1214-14 | Norway |
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Plutella xylostella | LEFIE003-10 | Finland |
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Plutella australiana | PHSAU1390-12 | Australia |
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Plutella australiana | MCCAA2949-12 | Australia |
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Plutella australiana | PHSAU1611-12 | Australia |
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Plutella australiana | PHSAU1612-12 | Australia |
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Plutella australiana | PHSAU1614-12 | Australia |
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Plutella australiana | PHSAU1615-12 | Australia |
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Eidophasia messingiella | LEFIG367-10 | Finland |
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Eidophasia messingiella | LEFIG368-10 | Finland |
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Eidophasia messingiella | LEFIE702-10 | Finland |
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Eidophasia vanella | LNAUT964-14 | United States |
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Eidophasia albidorsella | LNAUT960-14 | United States |
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Eidophasia albidorsella | LNAUT959-14 | United States |
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Eidophasia albidorsella | LNAUT961-14 | United States |
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Eidophasia albidorsella | LNAUT962-14 | United States |
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Eidophasia albidorsella | LNAUT963-14 | United States |
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Rhigognostis senilella | LON1272-14 | Norway |
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Rhigognostis senilella | ABOLA921-15 | Austria |
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Rhigognostis annulatella | LON220-08 | Norway |
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Rhigognostis annulatella | PHLAB866-10 | Italy |
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Rhigognostis schmaltzella | LEPVM047-12 | Norway |
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Rhigognostis schmaltzella | LEPVM048-12 | Norway |
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Interestingly, the two specimens representing P. haasi appear widely separated in the tree, and undoubtedly represent two different species.
The Maximum Likelihood tree based on DNA barcoding of P. polaris (arrow) and 10 other nominal species in the genus Plutella. Six species representing the two genera Eidophasia and Rhigognostis were used as outgroup. Bootstrap support values (1000 replicates) are listed above the branches. Number of specimens is given in brackets after each species.
The present results clearly demonstrate that P. polaris is a valid species, well defined morphologically, and based on genetic distances, is clearly delimited from other species in the genus. The new record from the Altai mountains in Russia, also demonstrates that the species has a much wider distribution (albeit with strongly divergent haplotypes) than has been recognized up to present. This solves the previous enigma of the existence of a prior endemic to Spitzbergen not collected for 142 years that existed close to the 80th parallel. More thorough collecting in northern Eurasia will most likely uncover new localities for the species. Another possibility is that P. polaris has a circumpolar distribution, as recently revealed for P. hyperboreella (
The genetic analysis also revealed a conflicting interpretation of the species P. haasi. The specimen forming a sister-group to P. hyperboreella originates from the type locality (Norway: Dovre), while the specimen located further towards the root of the Plutella clade, was collected in North Ural, Russia, and undoubtedly represents a new species. Further studies are needed to elucidate the status of these species.
The exact locality for Eaton’s first records of P. polaris in 1873 is not known, as the locality is simply referred to as “Wide Bay”, an old, unofficial name for Wijdefjorden, used up to 1934. As previously mentioned, Ringhorndalen is just one of several valleys opening towards the fjord, and Eaton may well have disembarked elsewhere along the fjord. From our own observations in Flatøyrdalen, the first valley south of Ringhorndalen, it is likely that the neighbouring valleys also may offer favourable conditions for the species. Hopefully future expeditions in the region will reveal a more detailed knowledge about the present distribution of P. polaris.
The Ringhorndal study was granted by the Svalbard Environmental Protection Fund (Svalbard miljøvernfond) (Ref.no. 14/108; RIS-10060). Our compliments go to Pernille B. Eidesen (project leader), Reidar Elven and Geir Arnesen for their kind teamwork and an exciting and memorable trip to Ringhorndalen in 2015. Karsten Sund (Natural History Museum, Oslo) is thanked for taking the photo of the specimen. We are grateful also to Marko Mutanen (Oulu, Finland) and Peter Huemer (Innsbruck, Austria) for giving access to their data, and in particular to P. Huemer for sharing the sequence of the Russian specimen of P. polaris.